Bench and industrial chemists use chromatography to separate a wanted analyte from a mixture. Since the 1920s, the accepted strategies to optimize a separation involve empirical methods and models. Now that pharmaceutical compounds are largely comprised of â€˜biologicâ€™ actives such as proteins, empirical optimization assigns a very high economic value to a process that most chemists take for granted. When the cost to bring one biologic to market is over two billion dollars, there is a strong incentive to reexamine our understanding of the interactions between analyte and stationary phase. The Landes Research Group seeks to relate the chemistry and physics of individual proteins interacting with a porous stationary support with newer, predictive models of chromatography. The interdisciplinary nature of our research group allows us to design new ways to study analyte-support interactions under the microscope and develop new theories towards a predictive, mechanistic description of chromatography.
Misiura, A., Shen, H., Tauzin, L., Dutta, C., Bishop, L. D. C., Carrejo, N. C., Zepeda O, J., Ramezani, S., Moringo, N. A., Marciel, A. B., Rossky, P. J., Landes, C. F., "Single-Molecule Dynamics Reflect IgG Conformational Changes Associated with Ion-Exchange Chromatography" Anal. Chem. 2021, 93, 32, 11200–11207
Bishop, L. D. C., Misiura, A., Landes, C.F., "A new metric for relating macroscopic chromatograms to microscopic surface dynamics: the distribution function ratio (DFR)" Analyst, 2021, 146, 4268-4279
Bishop, L. D. C., Misiura, A., Moringo, N. A., Landes, C. F. , "Unraveling peak asymmetry in chromatography through stochastictheory powered Monte Carlo simulations" J. Chromatogr. A 1625 (2020) 461323
Dutta, C., Bishop, L.D.C, Zepeda O, J., Chatterjee, S., Flatebo, C., Landes, C.F., "Imaging Switchable Protien Interactions With an Active Porous Polymer Support" J. Phys. Chem. B 2020, 124, 22, 4412-4420.