Predictive Separations

Predictive Separations

Predictive Separations

Bench and industrial chemists use chromatography to separate a wanted analyte from a mixture. Since the 1920s, the accepted strategies to optimize a separation involve empirical methods and models. Now that pharmaceutical compounds are largely comprised of ‘biologic’ actives such as proteins, empirical optimization assigns a very high economic value to a process that most chemists take for granted. When the cost to bring one biologic to market is over two billion dollars, there is a strong incentive to reexamine our understanding of the interactions between analyte and stationary phase. The Landes Research Group seeks to relate the chemistry and physics of individual proteins interacting with a porous stationary support with newer, predictive models of chromatography. The interdisciplinary nature of our research group allows us to design new ways to study analyte-support interactions under the microscope and develop new theories towards a predictive, mechanistic description of chromatography.

Approach

  • Chromatography is driven by chemical and physical interactions between the analyte and the stationary phase. We study processes such as protein adsorption and hindered transport within porous support materials. To do so with the appropriate time and space resolution often requires us to develop new types of microscopy and spectroscopy.
  • Previous theories used to translate single-molecule results to full elution profiles do not offer insights into the underlying chemistry of the chromatographic process. Developing a new numerical, holistic theory to accurately describe chromatography is an ongoing project in the Landes Research Group.

Moringo, N.A., Shen, H., Bishop, L.D.C., Wang, W., Landes, C.F., "Enhancing Analytical Separations Using Super-Resolution Microscopy" Annu. Rev. Phys. Chem. 2018, 69:14, 1-14.23

Moringo, N.A., Shen, H., Tauzin, L.J., Wang, W., Bishop, L.D.C., Landes, C.F., "Variable Lysozyme Transport Dynamics on Oxidatively Functionalized Polystyrene Films" Langmuir, 2017, 33(41), pp 10818-10828

Kisley, L.; Poongavanam, M. -V.; Kourentzi, K.; Willson, R.C.; Landes, C. F., "pH-dependence of single-protein adsorption and diffusion at a liquid chromatographic interface" J. Sep. Sci. 2015, 00, 1-7.