Bench and industrial chemists use chromatography to separate a wanted analyte from a mixture. Since the 1920s, the accepted strategies to optimize a separation involve empirical methods and models. Now that pharmaceutical compounds are largely comprised of ‘biologic’ actives such as proteins, empirical optimization assigns a very high economic value to a process that most chemists take for granted. When the cost to bring one biologic to market is over two billion dollars, there is a strong incentive to reexamine our understanding of the interactions between analyte and stationary phase. The Landes Research Group seeks to relate the chemistry and physics of individual proteins interacting with a porous stationary support with newer, predictive models of chromatography. The interdisciplinary nature of our research group allows us to design new ways to study analyte-support interactions under the microscope and develop new theories towards a predictive, mechanistic description of chromatography.
Bishop, L.D.C., Landes, C.F., "From a Protein's Perspective: Elution at the Single-Molecule Level" Acc. Chem. Res. 2018
Kisley, L.; Poongavanam, M. -V.; Kourentzi, K.; Willson, R.C.; Landes, C. F., "pH-dependence of single-protein adsorption and diffusion at a liquid chromatographic interface" J. Sep. Sci. 2015, 00, 1-7.
Kisley, L.; Landes, C. F., "Molecular Approaches to Chromatography Using Single Molecule Spectroscopy" Anal. Chem. 2015, 87,83-98.