Single-Molecule Spectroscopy

Single-Molecule Spectroscopy

Single-Molecule Spectroscopy

The primary goal of single-molecule spectroscopic studies is to detect and quantify the underlying populations that sum together to form the ensemble measurement. Interpreting the macroscopic observable in terms of microscopic measurements opens new opportunities for chemical tuning. Using single-molecule spectroscopy, we can track the interactions between protein and surface on a particle-by-particle basis, identify rare protein conformations that happen in every one out of a thousand molecules, and examine the statistical correlation between unique surface moeities and chemical behavior.


  • Surface hopping of single proteins was tracked using our in-house algorithm, Troika.
  • Combining single-molecule results and the stochastic theory of chromatography allowed our lab to shed light on the microscopic processes contributing to the separating action of chromatography.

Baiyasi, R., Goldwyn, H. J., McCarthy, L. A., West, C. A., Hosseini Jebeli, S. A., Masiello, D. J., Link, S., Landes, C. F., "Coupled-Dipole Modeling and Experimental Characterization of Geometry-Dependent Trochoidal Dichroism in Nanorod Trimers" ACS Photonics 2021, 8, 4, 1159–1168

Baiyasi, R., Gallagher, M.J., McCarthy, L.A., Searles, E.K., Zhaing, Q., Link, S., Landes, C.F., "Quantitative Analysis of Nanorod Aggregation and Morphology from Scanning Electron Micrographs Using SEMseg" J. Phys. Chem. A 2020, 124, 25, 5262–5270

Dutta, C., Bishop, L.D.C, Zepeda O, J., Chatterjee, S., Flatebo, C., Landes, C.F., "Imaging Switchable Protien Interactions With an Active Porous Polymer Support" J. Phys. Chem. B 2020, 124, 22, 4412-4420.

Ostovar, B., Su, M.N., Renard, D., Clark, B.D., Dongare, P.D., Dutta, C., Gross, N., Sader, J.E., Landes, C.F., Chang, W-S, Halas, N.J., Link, S., "Acoustic Vibrations of Al Nanocrystals: Size, Shape, and Crystallinity Revealed by Single-Particle Transient Extinction Spectroscopy" J. Phys. Chem. A 2020, 124, 19, 3924-3934.