Single-Molecule Spectroscopy

Single-Molecule Spectroscopy

Single-Molecule Spectroscopy

The primary goal of single-molecule spectroscopic studies is to detect and quantify the underlying populations that sum together to form the ensemble measurement. Interpreting the macroscopic observable in terms of microscopic measurements opens new opportunities for chemical tuning. Using single-molecule spectroscopy, we can track the interactions between protein and surface on a particle-by-particle basis, identify rare protein conformations that happen in every one out of a thousand molecules, and examine the statistical correlation between unique surface moeities and chemical behavior.


  • Surface hopping of single proteins was tracked using our in-house algorithm, Troika.
  • Combining single-molecule results and the stochastic theory of chromatography allowed our lab to shed light on the microscopic processes contributing to the separating action of chromatography.

Wang, W., Ye, F., Shen, H., Moringo, N.A., Dutta, C., Robinson, J.T., Landes, C.F., "Generalized method to design phase masks for 3D super-resolution microscopy" Opt. Express 2019, 27(3), pp. 3799-3816

Chatterjee, S., A., Carina, Nurik, C.E., Carrejo, N.C., Dutta, D., Jayaraman, V., Landes, C.F., "Phosphorylation Induces Conformational Rigidity at the C-Terminal Domain of AMPA Receptors" J. Phys. Chem. B 2019

Hoener, B.S., Kirchner, S.R., Heiderscheit, T.S., Collins, S.S.E., Chang, W., Link, S., Landes, C.F., "Plasmonic Sensing and Control of Single-Nanoparticle Electrochemistry" Chem. 2018, 4:7, 1560-1585

Moringo, N.A., Shen, H., Bishop, L.D.C., Wang, W., Landes, C.F., "Enhancing Analytical Separations Using Super-Resolution Microscopy" Annu. Rev. Phys. Chem. 2018, 69:14, 1-14.23